by Complete characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies.
- Missense mutations in LRRK2 (leucine-rich repeat kinase 2) are a serious explanation for PD (Parkinson’s illness). A number of antibodies towards Missense mutations in LRRK2 (leucine-rich repeat kinase 2) are a serious explanation for PD (Parkinson’s illness). A number of antibodies towards LRRK2 have been developed, however outcomes utilizing these polyclonal antibodies have diversified broadly resulting in conflicting conclusions. To handle this problem, the Michael J. Fox Basis for Parkinson’s Analysis generated a variety of monoclonal antibodies concentrating on epitopes throughout the LRRK2 protein.
- Within the current paper, we report optimized protocols and outcomes for ten monoclonal antibodies for immunoblotting, immunohistochemistry, immunoprecipitation and kinase exercise assays, in rat, mouse and human mind tissue. A number of efficacious antibodies have been recognized, however outcomes display that the mouse monoclonal N241A/34 is appropriate for many purposes, with the greatest total rabbit monoclonal antibody being c41-2.
Human Transmembrane protein 5, TMEM5 ELISA KIT
- These antibodies produced a dominant band of the anticipated dimension by way of immunoblotting and a scarcity of labelling in tissue derived from LRRK2-knockout animals beneath optimized circumstances. A big proportion of LRRK2 protein localizes to insoluble fractions and no proof of truncated LRRK2 protein was detected in any fraction from rodent or human tissues. An assay was developed for the sturdy detection of LRRK2 kinase exercise immediately from frozen mouse and human mind tissue, however precipitous declines in exercise have been noticed that corresponded to rising autopsy intervals and processing occasions. Lastly, we display the best ranges of brain-localized LRRK2 within the striatum, however notice differential expression patterns between rat and mouse in each striatum and cortex. Anti-LRRK2 monoclonal antibodies which might be limitless in availability along with the proposed standardized protocols ought to assist within the definition of LRRK2 operate in each well being and illness.have been developed, however outcomes utilizing these polyclonal antibodies have diversified broadly resulting in conflicting conclusions.
Polyclonal TMEM5 Antibody (Extracellular Domain)
 TMX1 Polyclonal Antibody |
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| 29996-50ul | SAB | 50ul | EUR 187 |
 Anti-TMX1 antibody |
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| STJ117135 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and one transmembrane domain. Unlike most members of this gene family, it lacks a C-terminal ER-retention sequence. The mature membrane-bound protein can both oxidize and reduce disulfide bonds and acts selectively on membrane-associated polypeptides. |
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Anti-TMX1 antibody |
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| STJ119397 | St John's Laboratory | 100 µl | EUR 277 |
 TMX1 siRNA |
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| 20-abx937556 | Abbexa |
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TMX1 siRNA |
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| 20-abx937557 | Abbexa |
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 TMX1 Polyclonal Conjugated Antibody |
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| C28770 | SAB | 100ul | EUR 397 |
TMX1 Polyclonal Conjugated Antibody |
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| C29996 | SAB | 100ul | EUR 397 |
 TMX1 Rabbit pAb |
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| A17219-100ul | Abclonal | 100 ul | EUR 308 |
TMX1 Rabbit pAb |
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| A17219-200ul | Abclonal | 200 ul | EUR 459 |
TMX1 Rabbit pAb |
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| A17219-20ul | Abclonal | 20 ul | EUR 183 |
TMX1 Rabbit pAb |
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| A17219-50ul | Abclonal | 50 ul | EUR 223 |
TMX1 Rabbit pAb |
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| A14936-100ul | Abclonal | 100 ul | EUR 308 |
TMX1 Rabbit pAb |
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| A14936-200ul | Abclonal | 200 ul | EUR 459 |
TMX1 Rabbit pAb |
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| A14936-20ul | Abclonal | 20 ul | EUR 183 |
TMX1 Rabbit pAb |
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| A14936-50ul | Abclonal | 50 ul | EUR 223 |
) Polyclonal TMX1 Antibody (C-term) |
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| AMM08249G | Leading Biology | 0.1ml | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TMX1 (C-term). This antibody is tested and proven to work in the following applications: |
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 Bovine TMX1 ELISA KIT |
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| ELI-28253b | Lifescience Market | 96 Tests | EUR 928 |
 Mouse Tmx1 ELISA KIT |
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| ELI-29414m | Lifescience Market | 96 Tests | EUR 865 |
 Human TMX1 ELISA KIT |
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| ELI-51830h | Lifescience Market | 96 Tests | EUR 824 |
 Mouse TMX1 shRNA Plasmid |
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| 20-abx977843 | Abbexa |
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 Human TMX1 shRNA Plasmid |
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| 20-abx962984 | Abbexa |
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) TMX1 Recombinant Protein (Rat) |
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| RP234011 | ABM | 100 ug | Ask for price |
) TMX1 Recombinant Protein (Human) |
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| RP044344 | ABM | 100 ug | Ask for price |
TMX1 Recombinant Protein (Human) |
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| RP032377 | ABM | 100 ug | Ask for price |
) TMX1 Recombinant Protein (Mouse) |
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| RP180140 | ABM | 100 ug | Ask for price |
 Polyclonal TMX1 antibody - C-terminal region |
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| AMM08250G | Leading Biology | 0.05mg | EUR 528 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TMX1 - C-terminal region. This antibody is tested and proven to work in the following applications: |
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 Antibody) Thioredoxin-Related Transmembrane Protein 1 (TMX1) Antibody |
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| abx034708-400ul | Abbexa | 400 ul | EUR 523 |
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Thioredoxin-Related Transmembrane Protein 1 (TMX1) Antibody |
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| abx034708-80l | Abbexa | 80 µl | EUR 286 |
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 (pORF)) Tmx1 ORF Vector (Rat) (pORF) |
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| ORF078005 | ABM | 1.0 ug DNA | EUR 506 |
 (pORF)) TMX1 ORF Vector (Human) (pORF) |
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| ORF014782 | ABM | 1.0 ug DNA | EUR 354 |
TMX1 ORF Vector (Human) (pORF) |
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| ORF010793 | ABM | 1.0 ug DNA | EUR 95 |
 (pORF)) Tmx1 ORF Vector (Mouse) (pORF) |
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| ORF060048 | ABM | 1.0 ug DNA | EUR 506 |
) Tmx1 sgRNA CRISPR Lentivector set (Rat) |
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| K6239901 | ABM | 3 x 1.0 ug | EUR 339 |
) Tmx1 sgRNA CRISPR Lentivector set (Mouse) |
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| K3696701 | ABM | 3 x 1.0 ug | EUR 339 |
) TMX1 sgRNA CRISPR Lentivector set (Human) |
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| K2413501 | ABM | 3 x 1.0 ug | EUR 339 |
 (Target 1)) Tmx1 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K6239902 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) Tmx1 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K6239903 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) Tmx1 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K6239904 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 1)) Tmx1 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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| K3696702 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) Tmx1 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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| K3696703 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) Tmx1 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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| K3696704 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 1)) TMX1 sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K2413502 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) TMX1 sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K2413503 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) TMX1 sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K2413504 | ABM | 1.0 ug DNA | EUR 154 |
 (pPB-C-His)) TMX1 Protein Vector (Rat) (pPB-C-His) |
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| PV312018 | ABM | 500 ng | EUR 603 |
 (pPB-N-His)) TMX1 Protein Vector (Rat) (pPB-N-His) |
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| PV312019 | ABM | 500 ng | EUR 603 |
 (pPM-C-HA)) TMX1 Protein Vector (Rat) (pPM-C-HA) |
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| PV312020 | ABM | 500 ng | EUR 603 |
 (pPM-C-His)) TMX1 Protein Vector (Rat) (pPM-C-His) |
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| PV312021 | ABM | 500 ng | EUR 603 |
 (pPB-C-His)) TMX1 Protein Vector (Human) (pPB-C-His) |
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| PV059125 | ABM | 500 ng | EUR 481 |
 (pPB-N-His)) TMX1 Protein Vector (Human) (pPB-N-His) |
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| PV059126 | ABM | 500 ng | EUR 481 |
 (pPM-C-HA)) TMX1 Protein Vector (Human) (pPM-C-HA) |
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| PV059127 | ABM | 500 ng | EUR 481 |
 (pPM-C-His)) TMX1 Protein Vector (Human) (pPM-C-His) |
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| PV059128 | ABM | 500 ng | EUR 481 |
 (pPB-C-His)) TMX1 Protein Vector (Mouse) (pPB-C-His) |
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| PV240190 | ABM | 500 ng | EUR 603 |
 (pPB-N-His)) TMX1 Protein Vector (Mouse) (pPB-N-His) |
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| PV240191 | ABM | 500 ng | EUR 603 |
 (pPM-C-HA)) TMX1 Protein Vector (Mouse) (pPM-C-HA) |
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| PV240192 | ABM | 500 ng | EUR 603 |
 (pPM-C-His)) TMX1 Protein Vector (Mouse) (pPM-C-His) |
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| PV240193 | ABM | 500 ng | EUR 603 |
- To handle this problem, the Michael J. Fox Basis for Parkinson’s Analysis generated a variety of monoclonal antibodies concentrating on epitopes throughout the LRRK2 protein.
Mouse Monoclonal Antibody to Human Topo IIB
- Within the current paper, we report optimized protocols and outcomes for ten monoclonal antibodies for immunoblotting, immunohistochemistry, immunoprecipitation and kinase exercise assays, in rat, mouse and human mind tissue. A number of efficacious antibodies have been recognized, however outcomes display that the mouse monoclonal N241A/34 is appropriate for many purposes, with the greatest total rabbit monoclonal antibody being c41-2.
- These antibodies produced a dominant band of the anticipated dimension by way of immunoblotting and a scarcity of labelling in tissue derived from LRRK2-knockout animals beneath optimized circumstances. A big proportion of LRRK2 protein localizes to insoluble fractions and no proof of truncated LRRK2 protein was detected in any fraction from rodent or human tissues. An assay was developed for the sturdy detection of LRRK2 kinase exercise immediately from frozen mouse and human mind tissue, however precipitous declines in exercise have been noticed that corresponded to rising autopsy intervals and processing occasions.
- Lastly, we display the best ranges of brain-localized LRRK2 within the striatum, however notice differential expression patterns between rat and mouse in each striatum and cortex. Anti–LRRK2 monoclonal antibodies which might be limitless in availability along with the proposed standardized protocols ought to assist within the definition of LRRK2 operate in each well being and illness.
 Antibody) Transmembrane Protein 5 (TMEM5) Antibody |
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| abx029014-80l | Abbexa | 80 µl | EUR 286 |
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Transmembrane Protein 5 (TMEM5) Antibody |
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| abx238783-100ug | Abbexa | 100 ug | EUR 509 |
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 Rabbit Polyclonal antibody Anti-CRBN |
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| Anti-CRBN | ImmunoStep | 50 µg | EUR 349 |
ELISA Kit) Human Transmembrane Protein 5 (TMEM5)ELISA Kit |
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| 201-12-2414 | SunredBio | 96 tests | EUR 440 |
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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
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 ELISA Kit) Human Transmembrane protein 5 (TMEM5) ELISA Kit |
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| abx383824-96tests | Abbexa | 96 tests | EUR 911 |
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 Human Transmembrane protein 5, TMEM5 ELISA KIT |
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| ELI-41877h | Lifescience Market | 96 Tests | EUR 824 |
ELISA Kit) Human Transmembrane Protein 5(TMEM5)ELISA Kit |
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| QY-E02427 | Qayee Biotechnology | 96T | EUR 361 |
 Mouse Transmembrane protein 5, Tmem5 ELISA KIT |
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| ELI-51691m | Lifescience Market | 96 Tests | EUR 865 |
ELISA Kit) Rat Transmembrane Protein 5(TMEM5)ELISA Kit |
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| QY-E10444 | Qayee Biotechnology | 96T | EUR 361 |
ELISA Kit) Mouse Transmembrane Protein 5(TMEM5)ELISA Kit |
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| QY-E21515 | Qayee Biotechnology | 96T | EUR 361 |
 anti- TMEM5 antibody |
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| FNab08783 | FN Test | 100µg | EUR 548.75 |
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Description: Antibody raised against TMEM5 |
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 Anti-TMEM5 antibody |
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| PAab08783 | Lifescience Market | 100 ug | EUR 386 |
) IL-8 (-5 to +5) |
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| 5-01377 | CHI Scientific | 4 x 5mg | Ask for price |
 TMEM5 antibody |
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| 20R-TR022 | Fitzgerald | 50 ug | EUR 656 |
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Description: Rabbit polyclonal TMEM5 antibody |
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TMEM5 antibody |
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| 20R-TR023 | Fitzgerald | 50 ug | EUR 656 |
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Description: Rabbit polyclonal TMEM5 antibody |
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TMEM5 antibody |
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| 20R-TR024 | Fitzgerald | 50 ug | EUR 656 |
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Description: Rabbit polyclonal TMEM5 antibody |
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 Rabbit Polyclonal Antibody to Human TDP1 |
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| TG2016 | TopoGen | 250 units | EUR 414 |
 or BCA Optima Protein Assay) BCA Protein Assay Ready to use blank pre-optimized strip plates (5) or BCA Optima Protein Assay |
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| BCAP-5 | Alpha Diagnostics | 1 pk | EUR 73 |
) Polyclonal TMEM5 Antibody (Extracellular Domain) |
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| APR02013G | Leading Biology | 0.05mg | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TMEM5 (Extracellular Domain). This antibody is tested and proven to work in the following applications: |
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 Rabbit Anti Human Interleukin-5 Polyclonal Antibody |
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| CPBT-65403RH | Creative Diagnostics | 0.1 mg | EUR 881 |
 Rabbit Anti Human Igfbp-5 Polyclonal Antibody |
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| CPBT-65632RH | Creative Diagnostics | 0.5 ml | EUR 819 |
 Rabbit Anti Human Caspase-5 Polyclonal Antibody |
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| CPBT-66285RH | Creative Diagnostics | 0.1 mg | EUR 580 |
 Rabbit Polyclonal Antibody to Human Topoisomerase IIb |
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| TG2010-3 | TopoGen | 250 units | EUR 414 |
 Rabbit Polyclonal Antibody to Human Topoisomerase IIIb |
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| TG2010-4 | TopoGen | 250 units | EUR 380 |
 Rabbit Polyclonal Antibody to Human Topoisomerase IIα |
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| TG2011-1 | TopoGen | 250 units | EUR 414 |
) Rabbit Polyclonal Antibody to Human Topoisomerase I (Rabbit Serum) |
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| TG2012-2 | TopoGen | 250 ul | EUR 380 |
 Rabbit Anti Human Interleukin-5 Polyclonal Antibody,Biotin |
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| CPBT-65402RH | Creative Diagnostics | 50 µg | EUR 881 |
 Polyclonal Antibody) Rabbit Anti 5-Lipoxygenase (Pser523) Polyclonal Antibody |
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| CPBT-65443R5 | Creative Diagnostics | 0.1 ml | EUR 985 |
 Rabbit Anti Mouse Mcp-5 Polyclonal Antibody |
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| CPBT-66985RM | Creative Diagnostics | 0.1 mg | EUR 881 |
 Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.serine 60-64 or 42-46 |
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| CPB-944RH | Creative Diagnostics | 100 ul | EUR 460 |
 Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.67-71 or 49-53 |
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| CPB-945RH | Creative Diagnostics | 100 ul | EUR 460 |
 Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.69-73 or 51-55 |
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| CPB-946RH | Creative Diagnostics | 100 ul | EUR 460 |
 Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.71-75 or 53-57 |
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| CPB-947RH | Creative Diagnostics | 100 ul | EUR 460 |
 Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.110-114 or 92-96 |
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| CPB-948RH | Creative Diagnostics | 100 ul | EUR 460 |
 Mouse Monoclonal Antibody to Human Topo IIB |
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| TG2011-5 | TopoGen | 100 units | EUR 380 |
) TMEM5 Recombinant Protein (Human) |
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| RP032194 | ABM | 100 ug | Ask for price |
 polyclonal antibody){kind=spacer} Myeloid/lymphoid or mixed-lineage leukemia translocated to 10 (MLLT10) polyclonal antibody |
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| ABP-PAB-10850 | Allele Biotech | 100 ug | Ask for price |
|
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 polyclonal antibody){kind=spacer} Myeloid/lymphoid or mixed-lineage leukemia translocated to 1 (MLLT1) polyclonal antibody |
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| ABP-PAB-10851 | Allele Biotech | 100 ug | Ask for price |
|
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 polyclonal antibody){kind=spacer} Myeloid/lymphoid or mixed-lineage leukemia translocated to 2 (MLLT2) polyclonal antibody |
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| ABP-PAB-10852 | Allele Biotech | 100 ug | Ask for price |
|
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 polyclonal antibody){kind=spacer} Myeloid/lymphoid or mixed-lineage leukemia translocated to 3 (MLLT3) polyclonal antibody |
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| ABP-PAB-10853 | Allele Biotech | 100 ug | Ask for price |
|
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 polyclonal antibody){kind=spacer} Myeloid/lymphoid or mixed-lineage leukemia translocated to 4 (MLLT4) polyclonal antibody |
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| ABP-PAB-10854 | Allele Biotech | 100 ug | Ask for price |
|
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 polyclonal antibody){kind=spacer} Myeloid/lymphoid or mixed-lineage leukemia translocated to 6 (MLLT6) polyclonal antibody |
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| ABP-PAB-10855 | Allele Biotech | 100 ug | Ask for price |
|
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 polyclonal antibody){kind=spacer} Myeloid/lymphoid or mixed-lineage leukemia translocated to 7 (MLLT7) polyclonal antibody |
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| ABP-PAB-10856 | Allele Biotech | 100 ug | Ask for price |
|
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 Polyclonal Antibody) Rabbit Anti-Human CFL1 (aa 1-5) Polyclonal Antibody |
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| CPB-1062RH | Creative Diagnostics | 100 ul | EUR 460 |
 Polyclonal Antibody) Rabbit Anti-Human MAP1LC3A (aa 5-10) Polyclonal Antibody |
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| CPB-1202RH | Creative Diagnostics | 100 ul | EUR 460 |
 Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
 Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
 Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280 |
 Rabbit Anti Mouse Mcp-5 Polyclonal Antibody,Biotin |
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| CPBT-66984RM | Creative Diagnostics | 50 µg | EUR 881 |
 Anti-Transmembrane protein 37 antibody |
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| STJ71308 | St John's Laboratory | 100 µg | EUR 260 |
 OR Antibody |
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| 44609-100ul | SAB | 100ul | EUR 252 |
OR Antibody |
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| 44609-50ul | SAB | 50ul | EUR 187 |
OR Antibody |
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| DF10250 | Affbiotech | 200ul | EUR 304 |
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Description: OR Antibody detects endogenous levels of total OR. |
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OR Antibody |
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| 20-abx217389 | Abbexa |
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OR Antibody |
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| ABD10250 | Lifescience Market | 100 ug | EUR 438 |
 polyclonal antibody) Myeloid/lymphoid or mixed-lineage leukemia 5 (MLL5) polyclonal antibody |
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| ABP-PAB-10849 | Allele Biotech | 100 ug | Ask for price |
|
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) 5-hmC polyclonal antibody (rabbit) |
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| 6830-50 | Biovision | EUR 316 | |
) IGFBP-5 Polyclonal Antibody (Rabbit) |
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| PAAF1 | GroPep | 200 µg | EUR 299 |
 Antibody) Lysosomal-Associated Transmembrane Protein 5 (LAPTM5) Antibody |
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| abx025582-400ul | Abbexa | 400 ul | EUR 523 |
|
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Lysosomal-Associated Transmembrane Protein 5 (LAPTM5) Antibody |
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| abx025582-80l | Abbexa | 80 µl | EUR 286 |
|
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Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies.
- The manufacturing of mouse peroxidase:antiperoxidase (PAP) complexes appropriate for immunohistological use along with monoclonal antibodies is described. Three approaches have been explored:
- 1) manufacturing of typical polyclonal PAP complexes;
Rabbit Anti-Human ATF2 Polyclonal Antibody
- 2) conversion of rabbit PAP to “pseudo-mouse PAP” by incubation with monoclonal mouse anti–rabbit immunoglobulin;
- 3) formation of PAP complexes from monoclonal mouse antiperoxidase. PAP complexes ready by the latter approach gave the greatest immunohistological labeling reactions, being secure on storage and appropriate with a variety of human monoclonal antibodies. Gel filtration revealed that monoclonal PAP is of decrease molecular weight than typical PAP complexes (fulfilling theoretical predictions primarily based on the monospecificity of monoclonal antibodies).
