FIG-1

Best Rabbit antibody to or anti-Human Polyclonal antibody

Complete characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies.

  • Missense mutations in LRRK2 (leucine-rich repeat kinase 2) are a serious explanation for PD (Parkinson’s illness). A number of antibodies towards Missense mutations in LRRK2 (leucine-rich repeat kinase 2) are a serious explanation for PD (Parkinson’s illness). A number of antibodies towards LRRK2 have been developed, however outcomes utilizing these polyclonal antibodies have diversified broadly resulting in conflicting conclusions. To handle this problem, the Michael J. Fox Basis for Parkinson’s Analysis generated a variety of monoclonal antibodies concentrating on epitopes throughout the LRRK2 protein.

 

  • Within the current paper, we report optimized protocols and outcomes for ten monoclonal antibodies for immunoblotting, immunohistochemistry, immunoprecipitation and kinase exercise assays, in rat, mouse and human mind tissue. A number of efficacious antibodies have been recognized, however outcomes display that the mouse monoclonal N241A/34 is appropriate for many purposes, with the greatest total rabbit monoclonal antibody being c41-2.

Human Transmembrane protein 5, TMEM5 ELISA KIT

FIG-1

  • These antibodies produced a dominant band of the anticipated dimension by way of immunoblotting and a scarcity of labelling in tissue derived from LRRK2-knockout animals beneath optimized circumstances. A big proportion of LRRK2 protein localizes to insoluble fractions and no proof of truncated LRRK2 protein was detected in any fraction from rodent or human tissues. An assay was developed for the sturdy detection of LRRK2 kinase exercise immediately from frozen mouse and human mind tissue, however precipitous declines in exercise have been noticed that corresponded to rising autopsy intervals and processing occasions. Lastly, we display the best ranges of brain-localized LRRK2 within the striatum, however notice differential expression patterns between rat and mouse in each striatum and cortex. Anti-LRRK2 monoclonal antibodies which might be limitless in availability along with the proposed standardized protocols ought to assist within the definition of LRRK2 operate in each well being and illness.have been developed, however outcomes utilizing these polyclonal antibodies have diversified broadly resulting in conflicting conclusions.

Polyclonal TMEM5 Antibody (Extracellular Domain)

TMX1 Polyclonal Antibody

29996-50ul 50ul
EUR 187

Anti-TMX1 antibody

STJ117135 100 µl
EUR 277
Description: This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and one transmembrane domain. Unlike most members of this gene family, it lacks a C-terminal ER-retention sequence. The mature membrane-bound protein can both oxidize and reduce disulfide bonds and acts selectively on membrane-associated polypeptides.

Anti-TMX1 antibody

STJ119397 100 µl
EUR 277

TMX1 siRNA

20-abx937556
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

TMX1 siRNA

20-abx937557
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

TMX1 Polyclonal Conjugated Antibody

C28770 100ul
EUR 397

TMX1 Polyclonal Conjugated Antibody

C29996 100ul
EUR 397

TMX1 Rabbit pAb

A17219-100ul 100 ul
EUR 308

TMX1 Rabbit pAb

A17219-200ul 200 ul
EUR 459

TMX1 Rabbit pAb

A17219-20ul 20 ul
EUR 183

TMX1 Rabbit pAb

A17219-50ul 50 ul
EUR 223

TMX1 Rabbit pAb

A14936-100ul 100 ul
EUR 308

TMX1 Rabbit pAb

A14936-200ul 200 ul
EUR 459

TMX1 Rabbit pAb

A14936-20ul 20 ul
EUR 183

TMX1 Rabbit pAb

A14936-50ul 50 ul
EUR 223

Polyclonal TMX1 Antibody (C-term)

AMM08249G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TMX1 (C-term). This antibody is tested and proven to work in the following applications:

Bovine TMX1 ELISA KIT

ELI-28253b 96 Tests
EUR 928

Mouse Tmx1 ELISA KIT

ELI-29414m 96 Tests
EUR 865

Human TMX1 ELISA KIT

ELI-51830h 96 Tests
EUR 824

Mouse TMX1 shRNA Plasmid

20-abx977843
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Human TMX1 shRNA Plasmid

20-abx962984
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

TMX1 Recombinant Protein (Rat)

RP234011 100 ug Ask for price

TMX1 Recombinant Protein (Human)

RP044344 100 ug Ask for price

TMX1 Recombinant Protein (Human)

RP032377 100 ug Ask for price

TMX1 Recombinant Protein (Mouse)

RP180140 100 ug Ask for price

Polyclonal TMX1 antibody - C-terminal region

AMM08250G 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TMX1 - C-terminal region. This antibody is tested and proven to work in the following applications:

Thioredoxin-Related Transmembrane Protein 1 (TMX1) Antibody

abx034708-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Thioredoxin-Related Transmembrane Protein 1 (TMX1) Antibody

abx034708-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Tmx1 ORF Vector (Rat) (pORF)

ORF078005 1.0 ug DNA
EUR 506

TMX1 ORF Vector (Human) (pORF)

ORF014782 1.0 ug DNA
EUR 354

TMX1 ORF Vector (Human) (pORF)

ORF010793 1.0 ug DNA
EUR 95

Tmx1 ORF Vector (Mouse) (pORF)

ORF060048 1.0 ug DNA
EUR 506

Tmx1 sgRNA CRISPR Lentivector set (Rat)

K6239901 3 x 1.0 ug
EUR 339

Tmx1 sgRNA CRISPR Lentivector set (Mouse)

K3696701 3 x 1.0 ug
EUR 339

TMX1 sgRNA CRISPR Lentivector set (Human)

K2413501 3 x 1.0 ug
EUR 339

Tmx1 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6239902 1.0 ug DNA
EUR 154

Tmx1 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6239903 1.0 ug DNA
EUR 154

Tmx1 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6239904 1.0 ug DNA
EUR 154

Tmx1 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3696702 1.0 ug DNA
EUR 154

Tmx1 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3696703 1.0 ug DNA
EUR 154

Tmx1 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3696704 1.0 ug DNA
EUR 154

TMX1 sgRNA CRISPR Lentivector (Human) (Target 1)

K2413502 1.0 ug DNA
EUR 154

TMX1 sgRNA CRISPR Lentivector (Human) (Target 2)

K2413503 1.0 ug DNA
EUR 154

TMX1 sgRNA CRISPR Lentivector (Human) (Target 3)

K2413504 1.0 ug DNA
EUR 154

TMX1 Protein Vector (Rat) (pPB-C-His)

PV312018 500 ng
EUR 603

TMX1 Protein Vector (Rat) (pPB-N-His)

PV312019 500 ng
EUR 603

TMX1 Protein Vector (Rat) (pPM-C-HA)

PV312020 500 ng
EUR 603

TMX1 Protein Vector (Rat) (pPM-C-His)

PV312021 500 ng
EUR 603

TMX1 Protein Vector (Human) (pPB-C-His)

PV059125 500 ng
EUR 481

TMX1 Protein Vector (Human) (pPB-N-His)

PV059126 500 ng
EUR 481

TMX1 Protein Vector (Human) (pPM-C-HA)

PV059127 500 ng
EUR 481

TMX1 Protein Vector (Human) (pPM-C-His)

PV059128 500 ng
EUR 481

TMX1 Protein Vector (Mouse) (pPB-C-His)

PV240190 500 ng
EUR 603

TMX1 Protein Vector (Mouse) (pPB-N-His)

PV240191 500 ng
EUR 603

TMX1 Protein Vector (Mouse) (pPM-C-HA)

PV240192 500 ng
EUR 603

TMX1 Protein Vector (Mouse) (pPM-C-His)

PV240193 500 ng
EUR 603
  • To handle this problem, the Michael J. Fox Basis for Parkinson’s Analysis generated a variety of monoclonal antibodies concentrating on epitopes throughout the LRRK2 protein.

 

Mouse Monoclonal Antibody to Human Topo IIB

 

  • Within the current paper, we report optimized protocols and outcomes for ten monoclonal antibodies for immunoblotting, immunohistochemistry, immunoprecipitation and kinase exercise assays, in rat, mouse and human mind tissue. A number of efficacious antibodies have been recognized, however outcomes display that the mouse monoclonal N241A/34 is appropriate for many purposes, with the greatest total rabbit monoclonal antibody being c41-2.

 

  • These antibodies produced a dominant band of the anticipated dimension by way of immunoblotting and a scarcity of labelling in tissue derived from LRRK2-knockout animals beneath optimized circumstances. A big proportion of LRRK2 protein localizes to insoluble fractions and no proof of truncated LRRK2 protein was detected in any fraction from rodent or human tissues. An assay was developed for the sturdy detection of LRRK2 kinase exercise immediately from frozen mouse and human mind tissue, however precipitous declines in exercise have been noticed that corresponded to rising autopsy intervals and processing occasions.

 

  • Lastly, we display the best ranges of brain-localized LRRK2 within the striatum, however notice differential expression patterns between rat and mouse in each striatum and cortex. AntiLRRK2 monoclonal antibodies which might be limitless in availability along with the proposed standardized protocols ought to assist within the definition of LRRK2 operate in each well being and illness.

 

 

Transmembrane Protein 5 (TMEM5) Antibody

abx029014-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Transmembrane Protein 5 (TMEM5) Antibody

abx238783-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Human Transmembrane Protein 5 (TMEM5)ELISA Kit

201-12-2414 96 tests
EUR 440
  • This Transmembrane Protein 5 ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Transmembrane protein 5 (TMEM5) ELISA Kit

abx383824-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

Human Transmembrane protein 5, TMEM5 ELISA KIT

ELI-41877h 96 Tests
EUR 824

Human Transmembrane Protein 5(TMEM5)ELISA Kit

QY-E02427 96T
EUR 361

Mouse Transmembrane protein 5, Tmem5 ELISA KIT

ELI-51691m 96 Tests
EUR 865

Rat Transmembrane Protein 5(TMEM5)ELISA Kit

QY-E10444 96T
EUR 361

Mouse Transmembrane Protein 5(TMEM5)ELISA Kit

QY-E21515 96T
EUR 361

anti- TMEM5 antibody

FNab08783 100µg
EUR 548.75
  • Immunogen: transmembrane protein 5
  • Uniprot ID: Q9Y2B1
  • Gene ID: 10329
  • Research Area: Cell Division and Proliferation
Description: Antibody raised against TMEM5

Anti-TMEM5 antibody

PAab08783 100 ug
EUR 386

IL-8 (-5 to +5)

5-01377 4 x 5mg Ask for price

TMEM5 antibody

20R-TR022 50 ug
EUR 656
Description: Rabbit polyclonal TMEM5 antibody

TMEM5 antibody

20R-TR023 50 ug
EUR 656
Description: Rabbit polyclonal TMEM5 antibody

TMEM5 antibody

20R-TR024 50 ug
EUR 656
Description: Rabbit polyclonal TMEM5 antibody

Rabbit Polyclonal Antibody to Human TDP1

TG2016 250 units
EUR 414

BCA Protein Assay Ready to use blank pre-optimized strip plates (5) or BCA Optima Protein Assay

BCAP-5 1 pk
EUR 73

Polyclonal TMEM5 Antibody (Extracellular Domain)

APR02013G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TMEM5 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Rabbit Anti Human Interleukin-5 Polyclonal Antibody

CPBT-65403RH 0.1 mg
EUR 881

Rabbit Anti Human Igfbp-5 Polyclonal Antibody

CPBT-65632RH 0.5 ml
EUR 819

Rabbit Anti Human Caspase-5 Polyclonal Antibody

CPBT-66285RH 0.1 mg
EUR 580

Rabbit Polyclonal Antibody to Human Topoisomerase IIb

TG2010-3 250 units
EUR 414

Rabbit Polyclonal Antibody to Human Topoisomerase IIIb

TG2010-4 250 units
EUR 380

Rabbit Polyclonal Antibody to Human Topoisomerase IIα

TG2011-1 250 units
EUR 414

Rabbit Polyclonal Antibody to Human Topoisomerase I (Rabbit Serum)

TG2012-2 250 ul
EUR 380

Rabbit Anti Human Interleukin-5 Polyclonal Antibody,Biotin

CPBT-65402RH 50 µg
EUR 881

Rabbit Anti 5-Lipoxygenase (Pser523) Polyclonal Antibody

CPBT-65443R5 0.1 ml
EUR 985

Rabbit Anti Mouse Mcp-5 Polyclonal Antibody

CPBT-66985RM 0.1 mg
EUR 881

Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.serine 60-64 or 42-46

CPB-944RH 100 ul
EUR 460

Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.67-71 or 49-53

CPB-945RH 100 ul
EUR 460

Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.69-73 or 51-55

CPB-946RH 100 ul
EUR 460

Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.71-75 or 53-57

CPB-947RH 100 ul
EUR 460

Rabbit Anti-Human ATF2 Polyclonal Antibody, aa.110-114 or 92-96

CPB-948RH 100 ul
EUR 460

Mouse Monoclonal Antibody to Human Topo IIB

TG2011-5 100 units
EUR 380

TMEM5 Recombinant Protein (Human)

RP032194 100 ug Ask for price

Myeloid/lymphoid or mixed-lineage leukemia translocated to 10 (MLLT10) polyclonal antibody

ABP-PAB-10850 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

Myeloid/lymphoid or mixed-lineage leukemia translocated to 1 (MLLT1) polyclonal antibody

ABP-PAB-10851 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

Myeloid/lymphoid or mixed-lineage leukemia translocated to 2 (MLLT2) polyclonal antibody

ABP-PAB-10852 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

Myeloid/lymphoid or mixed-lineage leukemia translocated to 3 (MLLT3) polyclonal antibody

ABP-PAB-10853 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

Myeloid/lymphoid or mixed-lineage leukemia translocated to 4 (MLLT4) polyclonal antibody

ABP-PAB-10854 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

Myeloid/lymphoid or mixed-lineage leukemia translocated to 6 (MLLT6) polyclonal antibody

ABP-PAB-10855 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

Myeloid/lymphoid or mixed-lineage leukemia translocated to 7 (MLLT7) polyclonal antibody

ABP-PAB-10856 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

Rabbit Anti-Human CFL1 (aa 1-5) Polyclonal Antibody

CPB-1062RH 100 ul
EUR 460

Rabbit Anti-Human MAP1LC3A (aa 5-10) Polyclonal Antibody

CPB-1202RH 100 ul
EUR 460

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Rabbit Anti Mouse Mcp-5 Polyclonal Antibody,Biotin

CPBT-66984RM 50 µg
EUR 881

Anti-Transmembrane protein 37 antibody

STJ71308 100 µg
EUR 260

OR Antibody

44609-100ul 100ul
EUR 252

OR Antibody

44609-50ul 50ul
EUR 187

OR Antibody

DF10250 200ul
EUR 304
Description: OR Antibody detects endogenous levels of total OR.

OR Antibody

20-abx217389
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

OR Antibody

ABD10250 100 ug
EUR 438

Myeloid/lymphoid or mixed-lineage leukemia 5 (MLL5) polyclonal antibody

ABP-PAB-10849 100 ug Ask for price
    • Product line: Oncoproteins
    • Brand:

5-hmC polyclonal antibody (rabbit)

6830-50
EUR 316

IGFBP-5 Polyclonal Antibody (Rabbit)

PAAF1 200 µg
EUR 299

Lysosomal-Associated Transmembrane Protein 5 (LAPTM5) Antibody

abx025582-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Lysosomal-Associated Transmembrane Protein 5 (LAPTM5) Antibody

abx025582-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

 

 

Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies.

 

  • The manufacturing of mouse peroxidase:antiperoxidase (PAP) complexes appropriate for immunohistological use along with monoclonal antibodies is described. Three approaches have been explored:
  • 1) manufacturing of typical polyclonal PAP complexes;

Rabbit Anti-Human ATF2 Polyclonal Antibody

  • 2) conversion of rabbit PAP to “pseudo-mouse PAP” by incubation with monoclonal mouse antirabbit immunoglobulin;

 

  • 3) formation of PAP complexes from monoclonal mouse antiperoxidase. PAP complexes ready by the latter approach gave the greatest immunohistological labeling reactions, being secure on storage and appropriate with a variety of human monoclonal antibodies. Gel filtration revealed that monoclonal PAP is of decrease molecular weight than typical PAP complexes (fulfilling theoretical predictions primarily based on the monospecificity of monoclonal antibodies).

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