Comparability of Business ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Primarily based Immunoassay for Detecting a Urine-Primarily based Bladder-Most cancers-Related Diagnostic Signature.
- The flexibility to precisely measure a number of proteins concurrently in a single assay has the potential to markedly enhance the effectivity of medical checks composed of a number of biomarkers. We investigated the diagnostic accuracy of the 2 multiplex protein array platforms for detecting a bladder-cancer-associated diagnostic signature in samples from a cohort of 80 topics (40 with bladder most cancers). Banked urine samples collected from Kyoto and Nara Universities have been in contrast to histologically decided bladder cancer.
- The concentrations of the 10 proteins (A1AT; apolipoprotein E-APOE; angiogenin-ANG; carbonic anhydrase 9-CA9; interleukin 8-IL-8; matrix metalloproteinase 9-MMP-9; matrix metalloproteinase 10-MMP10; plasminogen activator inhibitor 1-PAI-1; syndecan-SDC1; and vascular endothelial progress factor-VEGF) have been monitored utilizing two prototype multiplex array platforms and an enzyme-linked immunosorbent assay (ELISA) in keeping with the producer’s technical specs.
Rat Transmembrane Protein 27 (TMEM27) ELISA Kit
- The vary for detecting every biomarker was improved within the multiplex assays, regardless that the decrease restrict of quantification (LLOQ) was usually decrease within the business ELISA kits. The world underneath the receiver working traits (AUROC) of the prototype multiplex assays was reported to be 0.97 for the multiplex bead-based b(MBA) and 0.86 for the multiplex electrochemoluminescent assay (MEA).
- The sensitivities and specificities for MBA have been 0.93 and 0.95, respectively, and for MEA have been 0.85 and 0.80, respectively. Accuracy, optimistic predictive values (PPV), and unfavourable predictive values (NPV) for MBA have been 0.94, 0.95, and 0.93, respectively, and for MEA have been 0.83, 0.81, and 0.84, respectively. Primarily based on these encouraging preliminary information, we imagine {that a} multiplex protein array is a viable platform that may be utilized as an environment friendly and extremely correct software to quantitate a number of proteins inside biologic specimens.
Human Transmembrane Protein 27 (TMEM27) ELISA Kit
![]() Mouse Transmembrane Protein 27 (TMEM27) ELISA Kit |
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DLR-TMEM27-Mu-48T | DL Develop | 48T | EUR 527 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Transmembrane Protein 27 (TMEM27) in samples from tissue homogenates, cell lysates or other biological fluids. |
![]() Mouse Transmembrane Protein 27 (TMEM27) ELISA Kit |
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DLR-TMEM27-Mu-96T | DL Develop | 96T | EUR 688 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Transmembrane Protein 27 (TMEM27) in samples from tissue homogenates, cell lysates or other biological fluids. |
![]() Rat Transmembrane Protein 27 (TMEM27) ELISA Kit |
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DLR-TMEM27-Ra-48T | DL Develop | 48T | EUR 549 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Transmembrane Protein 27 (TMEM27) in samples from tissue homogenates or other biological fluids. |
![]() Rat Transmembrane Protein 27 (TMEM27) ELISA Kit |
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DLR-TMEM27-Ra-96T | DL Develop | 96T | EUR 718 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Transmembrane Protein 27 (TMEM27) in samples from tissue homogenates or other biological fluids. |
![]() Mouse Transmembrane Protein 27 (TMEM27) ELISA Kit |
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RD-TMEM27-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 533 |
![]() Mouse Transmembrane Protein 27 (TMEM27) ELISA Kit |
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RD-TMEM27-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 740 |
![]() Rat Transmembrane Protein 27 (TMEM27) ELISA Kit |
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RD-TMEM27-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 557 |
![]() Rat Transmembrane Protein 27 (TMEM27) ELISA Kit |
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RD-TMEM27-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 775 |
![]() Mouse Transmembrane Protein 27 (TMEM27) ELISA Kit |
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RDR-TMEM27-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 557 |
![]() Mouse Transmembrane Protein 27 (TMEM27) ELISA Kit |
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RDR-TMEM27-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 774 |
![]() Rat Transmembrane Protein 27 (TMEM27) ELISA Kit |
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RDR-TMEM27-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 583 |
![]() Rat Transmembrane Protein 27 (TMEM27) ELISA Kit |
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RDR-TMEM27-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 811 |
![]() Human Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 4731.5 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Transmembrane Protein 27 (TMEM27) in Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
![]() Human Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 477.3 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Transmembrane Protein 27 (TMEM27) in Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
![]() Human Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 639 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Transmembrane Protein 27 (TMEM27) in Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
![]() Human Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 2575.5 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Transmembrane Protein 27 (TMEM27) in Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
![]() Human Transmembrane Protein 27 (TMEM27) ELISA Kit |
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4-SEK477Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Transmembrane Protein 27 (TMEM27) in samples from Tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. |
![]() Human Transmembrane Protein 27 (TMEM27)ELISA Kit |
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201-12-2411 | SunredBio | 96 tests | EUR 440 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
![]() Human Transmembrane Protein 27 (TMEM27) ELISA Kit |
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20-abx153314 | Abbexa |
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![]() Human Transmembrane Protein 27(TMEM27)ELISA Kit |
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QY-E02429 | Qayee Biotechnology | 96T | EUR 361 |
![]() Human Transmembrane Protein 27 (TMEM27) Protein |
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20-abx069449 | Abbexa |
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![]() Recombinant Transmembrane Protein 27 (TMEM27) |
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4-RPK477Hu01 | Cloud-Clone |
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Description: Recombinant Human Transmembrane Protein 27 expressed in: E.coli |
![]() Recombinant Transmembrane Protein 27 (TMEM27) |
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4-RPK477Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Transmembrane Protein 27 expressed in: E.coli |
![]() Recombinant Transmembrane Protein 27 (TMEM27) |
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4-RPK477Mu02 | Cloud-Clone |
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Description: Recombinant Mouse Transmembrane Protein 27 expressed in: E.coli |
![]() Recombinant Transmembrane Protein 27 (TMEM27) |
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4-RPK477Ra01 | Cloud-Clone |
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Description: Recombinant Rat Transmembrane Protein 27 expressed in: E.coli |
![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx129603 | Abbexa |
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx129887 | Abbexa |
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx131398 | Abbexa |
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
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abx238776-100ug | Abbexa | 100 ug | EUR 509 |
![]() Transmembrane Protein 27 (TMEM27) Antibody |
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abx238777-100ug | Abbexa | 100 ug | EUR 551 |
![]() Transmembrane Protein 27 (TMEM27) Antibody |
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abx238778-100ug | Abbexa | 100 ug | EUR 551 |
![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx178706 | Abbexa |
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx174917 | Abbexa |
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx174918 | Abbexa |
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx301714 | Abbexa |
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx002497 | Abbexa |
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx102698 | Abbexa |
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
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20-abx132010 | Abbexa |
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![]() Dog Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Ca-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5386 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Transmembrane Protein 27 (TMEM27) in Tissue homogenates, cell lysates and other biological fluids. |
![]() Dog Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Ca-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 531.2 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Transmembrane Protein 27 (TMEM27) in Tissue homogenates, cell lysates and other biological fluids. |
![]() Dog Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Ca-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 716 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Transmembrane Protein 27 (TMEM27) in Tissue homogenates, cell lysates and other biological fluids. |
![]() Dog Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Ca-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 2922 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Transmembrane Protein 27 (TMEM27) in Tissue homogenates, cell lysates and other biological fluids. |
![]() Dog Transmembrane Protein 27 (TMEM27) ELISA Kit |
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4-SEK477Ca | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Dog Transmembrane Protein 27 (TMEM27) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. |
![]() Mouse Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Mu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 4862.4 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Transmembrane Protein 27 (TMEM27) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
![]() Mouse Transmembrane Protein 27 (TMEM27) ELISA Kit |
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SEK477Mu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 488.08 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Transmembrane Protein 27 (TMEM27) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Business Milk Enzyme-Linked Immunosorbent Assay (ELISA) Package Reactivities to Purified Milk Proteins and Milk-Derived Substances.
- Quite a few business enzyme-linked immunosorbent assay (ELISA) kits exist to quantitatively detect bovine milk residues in meals. Milk accommodates many proteins that may function ELISA targets together with caseins (α-, β-, or κ-casein) and whey proteins (α-lactalbumin or β-lactoglobulin).
- 9 commercially-available milk ELISA kits have been chosen to check the specificity and sensitivity with 5 purified milk proteins and three milk-derived elements.
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed
- The entire milk kits have been able to quantifying nonfat dry milk (NFDM), however didn’t essentially detect all particular person protein fractions. Whereas milk-derived elements have been detected by the kits, their quantitation could also be inaccurate as a consequence of using completely different calibrators, reference supplies, and antibodies in equipment growth.
- The institution of a typical reference materials for the calibration of milk ELISA kits is more and more essential. The suitable choice and understanding of milk ELISA kits for meals evaluation is critical to correct quantification of milk residues and knowledgeable threat management selections.
![]() Human Transmembrane Protein 5 (TMEM5)ELISA Kit |
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201-12-2414 | SunredBio | 96 tests | EUR 440 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
![]() Human Transmembrane protein 5 (TMEM5) ELISA Kit |
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abx383824-96tests | Abbexa | 96 tests | EUR 911 |
![]() Human Transmembrane Protein 5(TMEM5)ELISA Kit |
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QY-E02427 | Qayee Biotechnology | 96T | EUR 361 |
![]() Mouse Transmembrane protein 5, Tmem5 ELISA KIT |
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ELI-51691m | Lifescience Market | 96 Tests | EUR 865 |
![]() Rat Transmembrane Protein 5(TMEM5)ELISA Kit |
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QY-E10444 | Qayee Biotechnology | 96T | EUR 361 |
![]() Mouse Transmembrane Protein 5(TMEM5)ELISA Kit |
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QY-E21515 | Qayee Biotechnology | 96T | EUR 361 |
![]() TMEM5 ELISA KIT|Human |
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EF003685 | Lifescience Market | 96 Tests | EUR 689 |
![]() TMEM5 Recombinant Protein (Human) |
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RP032194 | ABM | 100 ug | Ask for price |
![]() TMEM5 antibody |
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20R-TR022 | Fitzgerald | 50 ug | EUR 656 |
Description: Rabbit polyclonal TMEM5 antibody |
![]() TMEM5 antibody |
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20R-TR023 | Fitzgerald | 50 ug | EUR 656 |
Description: Rabbit polyclonal TMEM5 antibody |
![]() TMEM5 antibody |
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20R-TR024 | Fitzgerald | 50 ug | EUR 656 |
Description: Rabbit polyclonal TMEM5 antibody |
![]() TMEM5 siRNA |
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20-abx937407 | Abbexa |
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![]() TMEM5 siRNA |
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20-abx937408 | Abbexa |
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![]() TMEM5 Recombinant Protein (Rat) |
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RP233807 | ABM | 100 ug | Ask for price |
![]() TMEM5 Recombinant Protein (Mouse) |
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RP179795 | ABM | 100 ug | Ask for price |
![]() Human TMEM5 shRNA Plasmid |
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20-abx957002 | Abbexa |
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![]() Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed |
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ELISA-1 | Alpha Diagnostics | 1 | EUR 202 |
![]() Anti-TMEM5 antibody |
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PAab08783 | Lifescience Market | 100 ug | EUR 386 |
![]() TMEM5 cloning plasmid |
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CSB-CL023848HU-10ug | Cusabio | 10ug | EUR 482 |
Description: A cloning plasmid for the TMEM5 gene. |
![]() anti- TMEM5 antibody |
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FNab08783 | FN Test | 100µg | EUR 548.75 |
Description: Antibody raised against TMEM5 |
![]() Human Transmembrane channel- like protein 5, TMC5 ELISA KIT |
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ELI-45963h | Lifescience Market | 96 Tests | EUR 824 |
![]() TMEM5 ORF Vector (Human) (pORF) |
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ORF010732 | ABM | 1.0 ug DNA | EUR 95 |
![]() TMEM5 Protein Vector (Human) (pPB-C-His) |
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PV042925 | ABM | 500 ng | EUR 329 |
![]() TMEM5 Protein Vector (Human) (pPB-N-His) |
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PV042926 | ABM | 500 ng | EUR 329 |
![]() TMEM5 Protein Vector (Human) (pPM-C-HA) |
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PV042927 | ABM | 500 ng | EUR 329 |
![]() TMEM5 Protein Vector (Human) (pPM-C-His) |
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PV042928 | ABM | 500 ng | EUR 329 |
![]() Human Transmembrane protease serine 5, TMPRSS5 ELISA KIT |
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ELI-41888h | Lifescience Market | 96 Tests | EUR 824 |
![]() Human Transmembrane Protease, Serine 5 (TMPRSS5) ELISA Kit |
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abx383843-96tests | Abbexa | 96 tests | EUR 911 |
![]() Recombinant human Transmembrane emp24 domain-containing protein 5 |
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P1796 | FN Test | 100ug | Ask for price |
Description: Recombinant protein for human Transmembrane emp24 domain-containing protein 5 |
![]() Mouse TMEM5 shRNA Plasmid |
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20-abx980998 | Abbexa |
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![]() TMEM5 sgRNA CRISPR Lentivector set (Human) |
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K2386501 | ABM | 3 x 1.0 ug | EUR 339 |
![]() Mouse Transmembrane channel- like protein 5, Tmc5 ELISA KIT |
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ELI-29403m | Lifescience Market | 96 Tests | EUR 865 |
![]() Interferon-Induced Transmembrane Protein 5 (IFITM5) Antibody |
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20-abx312820 | Abbexa |
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![]() Lysosomal-Associated Transmembrane Protein 5 (LAPTM5) Antibody |
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20-abx307047 | Abbexa |
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![]() Lysosomal-Associated Transmembrane Protein 5 (LAPTM5) Antibody |
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abx025582-400ul | Abbexa | 400 ul | EUR 523 |
![]() Lysosomal-Associated Transmembrane Protein 5 (LAPTM5) Antibody |
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abx025582-80l | Abbexa | 80 µl | EUR 286 |
![]() Interferon-Induced Transmembrane Protein 5 (IFITM5) Antibody |
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abx026061-400ul | Abbexa | 400 ul | EUR 523 |
![]() Interferon-Induced Transmembrane Protein 5 (IFITM5) Antibody |
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abx026061-80l | Abbexa | 80 µl | EUR 286 |
![]() 5-Formylcytosine (5-fC) ELISA Kit |
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MET-5102-5 | Cell Biolabs | 5 x 96 assays | EUR 2283 |
![]() 5-Carboxylcytosine (5-caC) ELISA Kit |
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MET-5103-5 | Cell Biolabs | 5 x 96 assays | EUR 2283 |
![]() TMEM5 Protein Vector (Rat) (pPB-C-His) |
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PV311746 | ABM | 500 ng | EUR 603 |
![]() TMEM5 Protein Vector (Rat) (pPB-N-His) |
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PV311747 | ABM | 500 ng | EUR 603 |
![]() TMEM5 Protein Vector (Rat) (pPM-C-HA) |
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PV311748 | ABM | 500 ng | EUR 603 |
![]() TMEM5 Protein Vector (Rat) (pPM-C-His) |
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PV311749 | ABM | 500 ng | EUR 603 |
![]() TMEM5 Protein Vector (Mouse) (pPB-C-His) |
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PV239730 | ABM | 500 ng | EUR 603 |
![]() TMEM5 Protein Vector (Mouse) (pPB-N-His) |
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PV239731 | ABM | 500 ng | EUR 603 |
![]() TMEM5 Protein Vector (Mouse) (pPM-C-HA) |
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PV239732 | ABM | 500 ng | EUR 603 |
![]() TMEM5 Protein Vector (Mouse) (pPM-C-His) |
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PV239733 | ABM | 500 ng | EUR 603 |
![]() Tmem5 ORF Vector (Rat) (pORF) |
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ORF077937 | ABM | 1.0 ug DNA | EUR 506 |
- The mouse bioassay (MBA) is the one accepted customary method for detection of botulinum neurotoxins (BoNTs) in meals. The ELISA methodology has a number of benefits over the MBA and is due to this fact extensively used for in vitro detection of BoNTs.
Human Transmembrane channel- like protein 5, TMC5 ELISA KIT
- The US Meals and Drug Administration (FDA) and the Facilities for Illness Management and Prevention (CDC) carried out a precollaborative examine to judge the applicability of Botulinum Toxin ELISA kits for the detection of BoNT serotypes A, B, E, and F in a wide range of meals matrices.
- On this examine, meals samples (e.g., broccoli, salami, smoked salmon, inexperienced beans, orange juice, tomato juice, low-fat plain yogurt, entire milk, liquid toddler formulation milk, and liquid eggs) have been spiked with excessive, medium, and low focus BoNT serotypes A, B, E, and F. Samples (unspiked and spiked) have been examined at each laboratories by the ELISA kits.
Human Transmembrane Protease, Serine 5 (TMPRSS5) ELISA Kit
- All meals samples have been positive for BoNTs by ELISA in each laboratories at medium and excessive spiking ranges; a optimistic ELISA end in low spiked samples was each serotype and laboratory dependent. Total, the ELISA methodology seems to be an efficient preliminary screening methodology for BoNT detection in meals matrices.