Heterologous expression of fused genes encoding the glycoprotein 5 from PRRSV: a means for producing practical protein in prokaryotic microorganism.
-
OBJECTIVETo acquire fbpB-esxA fusing gene of Mycobacterium tuberculosis (MTB), categorical the encoded fusing protein in Escherichia coli (E. coli), establish protein acquired, and predict the construction and performance of the protein using strategies of bioinformatics.
-
METHODSfbpB and esxA gene had been amplified from genome of MTB H37Rv by PCR. The fbpB–esxA fusing gene ligated by (Gly(4)Ser)(3) linker was gained by way of Gene Splicing by Overlapping Extension PCR (SOE-PCR), and fusing gene was cloned into expression vector pET-30a. The recombinant plasmid was sequenced and expressed in E. coli BL21(DE3). The protein was recognized by Western blot utilizing anti-HIS antibody. Secondary construction and antigenic epitopes of the protein had been predicting utilizing instruments of bioinformatics.
Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody
-
RESULTSThe DNA sequences of fbpB–esxA had been an identical with that revealed by GenBank. The Ag85B-ESAT-6 fusion protein about 50 kDa comprised 485 amino acids was effectively produced from expression system in E. coli BL21(DE3) underneath the induction of IPTG. Bioinformatics evaluation confirmed the protein contained one transmembrane area and fourteen potential antigenic epitopes.
-
CONCLUSIONSThe Ag85B-ESAT-6 fusion protein is efficiently expressed with N-terminal HIS-tag. Gel filtration demonstrated that it exists as insoluble inclusion our bodies primarily. The existence of linker would not have an effect on immunogenicity of Ag85B and ESAT-6. It’s going to permit for characterization in vitro and set up a basis of additional operate analysis similar to vaccine or diagnostic reagent.
Transmembrane Protein 132A (TMEM132A) Antibody

![]() pETG- 30A |
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PVT10594 | Lifescience Market | 2 ug | EUR 370 |
![]() miRZip-30a anti-miR-30a microRNA construct |
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MZIP30a-PA-1 | SBI | Bacterial Streak | EUR 684 |
![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody |
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20-abx318608 | Abbexa |
|
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![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody |
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abx239079-100ug | Abbexa | 100 ug | EUR 509 |
![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody |
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abx145931-100ug | Abbexa | 100 ug | EUR 391 |
![]() pET- 30a Plasmid |
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PVT0343 | Lifescience Market | 2 ug | EUR 241 |
![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody (HRP) |
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20-abx308461 | Abbexa |
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![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody (FITC) |
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20-abx308462 | Abbexa |
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![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody (Biotin) |
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20-abx308463 | Abbexa |
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![]() rno-miR-30a Primers |
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MP-r00070 | ABM | 150 ul / 10 uM | EUR 176 |
![]() hsa-miR-30a Primers |
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MPH01324 | ABM | 150 ul / 10 uM | EUR 121 |
![]() hsa-miR-30a* Primers |
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MPH02040 | ABM | 150 ul / 10 uM | EUR 121 |
![]() mmu-miR-30a Primers |
|||
MPM00359 | ABM | 150 ul / 10 uM | EUR 121 |
![]() Ankyrin Repeat Domain-Containing Protein 30A (AN30A) Antibody |
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20-abx119569 | Abbexa |
|
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![]() Ankyrin Repeat Domain-Containing Protein 30A (ANKRD30A) Antibody |
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20-abx121268 | Abbexa |
|
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![]() Ankyrin repeat domain-containing protein 30A (ANKRD30A) Antibody |
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20-abx324735 | Abbexa |
|
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![]() Chicken IgA ELISA Kit |
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E-30A | Immunology Consultants Laboratory | 1 x 96 well plate | EUR 395 |
![]() Custom Anti-Peptide antibodies (peptide synth. ~30-aa, conjug., 2 rabbits, ELISA & Aff. Purif.) |
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ABPEP-30A | Alpha Diagnostics | 1 | EUR 2716 |
![]() hsa-miR-30a miRNA Inhibitor |
|||
MIH01689 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() mmu-miR-30a-3p Primers |
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MPM01155 | ABM | 150 ul / 10 uM | EUR 121 |
![]() mmu-miR-30a-5p Primers |
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MPM01156 | ABM | 150 ul / 10 uM | EUR 121 |
![]() rno-miR-30a-3p Primers |
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MPR00593 | ABM | 150 ul / 10 uM | EUR 176 |
![]() rno-miR-30a-5p Primers |
|||
MPR00594 | ABM | 150 ul / 10 uM | EUR 176 |
![]() rno??miR-30a miRNA Antagomir |
|||
MNR01425 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() hsa-miR-30a-3p Primers |
|||
MPH02410 | ABM | 150 ul / 10 uM | EUR 121 |
![]() hsa-miR-30a-5p Primers |
|||
MPH02411 | ABM | 150 ul / 10 uM | EUR 121 |
![]() hsa-miR-30a miRNA Antagomir |
|||
MNH01689 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a miRNA Antagomir |
|||
MNM01777 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a miRNA Inhibitor |
|||
MIM01777 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() rno??miR-30a miRNA Inhibitor |
|||
MIR01425 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() Transmembrane protein 147 antibody |
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23105-100ul | SAB | 100ul | EUR 390 |
![]() Transmembrane protein 2 Antibody |
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48031-100ul | SAB | 100ul | EUR 333 |
![]() Transmembrane protein 2 Antibody |
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48031-50ul | SAB | 50ul | EUR 239 |
![]() Transmembrane protein 132A Antibody |
|||
48033-100ul | SAB | 100ul | EUR 333 |
![]() Transmembrane protein 132A Antibody |
|||
48033-50ul | SAB | 50ul | EUR 239 |
![]() Transmembrane protein 200A Antibody |
|||
48081-100ul | SAB | 100ul | EUR 333 |
![]() Transmembrane protein 200A Antibody |
|||
48081-50ul | SAB | 50ul | EUR 239 |
![]() Bovine Tetratricopeptide repeat protein 30A, TTC30A ELISA KIT |
|||
ELI-40057b | Lifescience Market | 96 Tests | EUR 928 |
![]() Human Tetratricopeptide repeat protein 30A, TTC30A ELISA KIT |
|||
ELI-40191h | Lifescience Market | 96 Tests | EUR 824 |
![]() Human Tetratricopeptide Repeat Protein 30A (TTC30A) ELISA Kit |
|||
abx384009-96tests | Abbexa | 96 tests | EUR 911 |
![]() Recombinant human Ankyrin repeat domain-containing protein 30A |
|||
P1578 | FN Test | 100ug | Ask for price |
Description: Recombinant protein for human Ankyrin repeat domain-containing protein 30A |
![]() hsa-miR-30a-3p miRNA Inhibitor |
|||
MIH01690 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() hsa-miR-30a-5p miRNA Inhibitor |
|||
MIH01691 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() rno-miR-30a-3p miRNA Antagomir |
|||
MNR01426 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() rno-miR-30a-5p miRNA Antagomir |
|||
MNR01427 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() hsa-miR-30a-3p miRNA Antagomir |
|||
MNH01690 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() hsa-miR-30a-5p miRNA Antagomir |
|||
MNH01691 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a-3p miRNA Antagomir |
|||
MNM01778 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a-5p miRNA Antagomir |
|||
MNM01779 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a-3p miRNA Inhibitor |
|||
MIM01778 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() mmu-miR-30a-5p miRNA Inhibitor |
|||
MIM01779 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() rno-miR-30a-3p miRNA Inhibitor |
|||
MIR01426 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() rno-miR-30a-5p miRNA Inhibitor |
|||
MIR01427 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() Transmembrane protein 14B Antibody (Biotin) |
|||
20-abx106002 | Abbexa |
|
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![]() Transmembrane protein 14B Antibody (FITC) |
|||
20-abx107416 | Abbexa |
|
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![]() Transmembrane Protein 74 (TMEM74) Antibody |
|||
20-abx126713 | Abbexa |
|
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
|||
20-abx129603 | Abbexa |
|
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![]() Transmembrane Protein 173 (TMEM173) Antibody |
|||
20-abx129806 | Abbexa |
|
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![]() Transmembrane Protein 27 (TMEM27) Antibody |
|||
20-abx129887 | Abbexa |
|
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![]() Transmembrane Protein 1 (TMEM1) Antibody |
|||
20-abx130992 | Abbexa |
|
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Fractalkine and CX3CR1 mediate a novel mechanism of leukocyte seize, agency adhesion, and activation underneath physiologic circulate.
- The herpesvirus entry mediator C (HveC), beforehand referred to as poliovirus receptor-related protein 1 (PRR1), and the herpesvirus Ig-like receptor (HIgR) are the bona fide receptors employed by herpes simplex virus-1 and -2 (HSV-1 and -2) for entry into the human cell traces most often utilized in HSV research. They share an an identical ectodomain made of 1 V and two C2 domains and differ in transmembrane and cytoplasmic areas.
Ankyrin repeat domain-containing protein 30A (ANKRD30A) Antibody
- Expression of their mRNA within the human nervous system suggests potential utilization of those receptors in people within the path of neuron an infection by HSV. Glyco<em>protein</em> D (gD) is the virion element that mediates HSV-1 entry into cells by interplay with mobile receptors. We report on the identification of the V area of HIgR/PRR1 as a significant practical area in HSV-1 entry by a number of approaches. First, the epitope acknowledged by mAb R1.
![]() Mouse pre-microRNA Expression Construct mir-30a |
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MMIR-30a-PA-1 | SBI | Bacterial Streak | EUR 684 |
![]() pET- 30a |
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PVT0084 | Lifescience Market | 2 ug | EUR 256 |
![]() pETG- 30A |
|||
PVT10594 | Lifescience Market | 2 ug | EUR 370 |
![]() miRZip-30a anti-miR-30a microRNA construct |
|||
MZIP30a-PA-1 | SBI | Bacterial Streak | EUR 684 |
![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody |
|||
20-abx318608 | Abbexa |
|
|
![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody |
|||
abx239079-100ug | Abbexa | 100 ug | EUR 509 |
![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody |
|||
abx145931-100ug | Abbexa | 100 ug | EUR 391 |
![]() pET- 30a Plasmid |
|||
PVT0343 | Lifescience Market | 2 ug | EUR 241 |
![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody (HRP) |
|||
20-abx308461 | Abbexa |
|
|
![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody (FITC) |
|||
20-abx308462 | Abbexa |
|
|
![]() Tetratricopeptide Repeat Protein 30A (TTC30A) Antibody (Biotin) |
|||
20-abx308463 | Abbexa |
|
|
![]() rno-miR-30a Primers |
|||
MP-r00070 | ABM | 150 ul / 10 uM | EUR 176 |
![]() hsa-miR-30a Primers |
|||
MPH01324 | ABM | 150 ul / 10 uM | EUR 121 |
![]() hsa-miR-30a* Primers |
|||
MPH02040 | ABM | 150 ul / 10 uM | EUR 121 |
![]() mmu-miR-30a Primers |
|||
MPM00359 | ABM | 150 ul / 10 uM | EUR 121 |
![]() Ankyrin Repeat Domain-Containing Protein 30A (AN30A) Antibody |
|||
20-abx119569 | Abbexa |
|
|
![]() Ankyrin Repeat Domain-Containing Protein 30A (ANKRD30A) Antibody |
|||
20-abx121268 | Abbexa |
|
|
![]() Ankyrin repeat domain-containing protein 30A (ANKRD30A) Antibody |
|||
20-abx324735 | Abbexa |
|
|
![]() Chicken IgA ELISA Kit |
|||
E-30A | Immunology Consultants Laboratory | 1 x 96 well plate | EUR 395 |
![]() Custom Anti-Peptide antibodies (peptide synth. ~30-aa, conjug., 2 rabbits, ELISA & Aff. Purif.) |
|||
ABPEP-30A | Alpha Diagnostics | 1 | EUR 2716 |
![]() hsa-miR-30a miRNA Inhibitor |
|||
MIH01689 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() mmu-miR-30a-3p Primers |
|||
MPM01155 | ABM | 150 ul / 10 uM | EUR 121 |
![]() mmu-miR-30a-5p Primers |
|||
MPM01156 | ABM | 150 ul / 10 uM | EUR 121 |
![]() rno-miR-30a-3p Primers |
|||
MPR00593 | ABM | 150 ul / 10 uM | EUR 176 |
![]() rno-miR-30a-5p Primers |
|||
MPR00594 | ABM | 150 ul / 10 uM | EUR 176 |
![]() rno??miR-30a miRNA Antagomir |
|||
MNR01425 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() hsa-miR-30a-3p Primers |
|||
MPH02410 | ABM | 150 ul / 10 uM | EUR 121 |
![]() hsa-miR-30a-5p Primers |
|||
MPH02411 | ABM | 150 ul / 10 uM | EUR 121 |
![]() hsa-miR-30a miRNA Antagomir |
|||
MNH01689 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a miRNA Antagomir |
|||
MNM01777 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a miRNA Inhibitor |
|||
MIM01777 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() rno??miR-30a miRNA Inhibitor |
|||
MIR01425 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() Transmembrane protein 147 antibody |
|||
23105-100ul | SAB | 100ul | EUR 390 |
![]() Transmembrane protein 2 Antibody |
|||
48031-100ul | SAB | 100ul | EUR 333 |
![]() Transmembrane protein 2 Antibody |
|||
48031-50ul | SAB | 50ul | EUR 239 |
![]() Transmembrane protein 132A Antibody |
|||
48033-100ul | SAB | 100ul | EUR 333 |
![]() Transmembrane protein 132A Antibody |
|||
48033-50ul | SAB | 50ul | EUR 239 |
![]() Transmembrane protein 200A Antibody |
|||
48081-100ul | SAB | 100ul | EUR 333 |
![]() Transmembrane protein 200A Antibody |
|||
48081-50ul | SAB | 50ul | EUR 239 |
![]() Bovine Tetratricopeptide repeat protein 30A, TTC30A ELISA KIT |
|||
ELI-40057b | Lifescience Market | 96 Tests | EUR 928 |
![]() Human Tetratricopeptide repeat protein 30A, TTC30A ELISA KIT |
|||
ELI-40191h | Lifescience Market | 96 Tests | EUR 824 |
![]() Human Tetratricopeptide Repeat Protein 30A (TTC30A) ELISA Kit |
|||
abx384009-96tests | Abbexa | 96 tests | EUR 911 |
![]() Recombinant human Ankyrin repeat domain-containing protein 30A |
|||
P1578 | FN Test | 100ug | Ask for price |
Description: Recombinant protein for human Ankyrin repeat domain-containing protein 30A |
![]() hsa-miR-30a-3p miRNA Inhibitor |
|||
MIH01690 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() hsa-miR-30a-5p miRNA Inhibitor |
|||
MIH01691 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() rno-miR-30a-3p miRNA Antagomir |
|||
MNR01426 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() rno-miR-30a-5p miRNA Antagomir |
|||
MNR01427 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() hsa-miR-30a-3p miRNA Antagomir |
|||
MNH01690 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() hsa-miR-30a-5p miRNA Antagomir |
|||
MNH01691 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a-3p miRNA Antagomir |
|||
MNM01778 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a-5p miRNA Antagomir |
|||
MNM01779 | ABM | 2 x 5.0 nmol | EUR 329 |
![]() mmu-miR-30a-3p miRNA Inhibitor |
|||
MIM01778 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() mmu-miR-30a-5p miRNA Inhibitor |
|||
MIM01779 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() rno-miR-30a-3p miRNA Inhibitor |
|||
MIR01426 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() rno-miR-30a-5p miRNA Inhibitor |
|||
MIR01427 | ABM | 2 x 5.0 nmol | EUR 176 |
![]() Transmembrane protein 14B Antibody (Biotin) |
|||
20-abx106002 | Abbexa |
|
|
![]() Transmembrane protein 14B Antibody (FITC) |
|||
20-abx107416 | Abbexa |
|
|
![]() Transmembrane Protein 74 (TMEM74) Antibody |
|||
20-abx126713 | Abbexa |
|
|
![]() Transmembrane Protein 27 (TMEM27) Antibody |
|||
20-abx129603 | Abbexa |
|
|
![]() Transmembrane Protein 173 (TMEM173) Antibody |
|||
20-abx129806 | Abbexa |
|
|
![]() Transmembrane Protein 27 (TMEM27) Antibody |
|||
20-abx129887 | Abbexa |
|
|
![]() Transmembrane Protein 1 (TMEM1) Antibody |
|||
20-abx130992 | Abbexa |
|
|
![]() Transmembrane Protein 1 (TMEM1) Antibody |
|||
20-abx130993 | Abbexa |
|
|
![]() Transmembrane Protein 27 (TMEM27) Antibody |
|||
20-abx131398 | Abbexa |
|
|
![]() Transmembrane Protein 106B (TMEM106B) Antibody |
|||
20-abx116234 | Abbexa |
|
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![]() Transmembrane Protein 108 (TMEM108) Antibody |
|||
20-abx116235 | Abbexa |
|
|
![]() Transmembrane Protein 11 (TMEM11) Antibody |
|||
20-abx116236 | Abbexa |
|
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![]() Transmembrane Protein 120A (TMEM120A) Antibody |
|||
20-abx116237 | Abbexa |
|
|
![]() Transmembrane Protein 141 (TMEM141) Antibody |
|||
20-abx116238 | Abbexa |
|
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![]() Transmembrane Protein 165 (TMEM165) Antibody |
|||
20-abx116239 | Abbexa |
|
|
![]() Transmembrane Protein 173 (TMEM173) Antibody |
|||
20-abx116240 | Abbexa |
|
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![]() Transmembrane Protein 175 (THEM175) Antibody |
|||
20-abx116241 | Abbexa |
|
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![]() Transmembrane Protein 176A (THEM176A) Antibody |
|||
20-abx116242 | Abbexa |
|
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![]() Transmembrane Protein 176B (THEM176B) Antibody |
|||
20-abx116243 | Abbexa |
|
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![]() Transmembrane Protein 27 (THEM27) Antibody |
|||
20-abx116244 | Abbexa |
|
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![]() Transmembrane Protein 38A (THEM38A) Antibody |
|||
20-abx116245 | Abbexa |
|
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![]() Transmembrane Protein 41A (THEM41A) Antibody |
|||
20-abx116246 | Abbexa |
|
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![]() Transmembrane Protein 50B (THEM50B) Antibody |
|||
20-abx116247 | Abbexa |
|
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![]() Transmembrane Protein 67 (THEM67) Antibody |
|||
20-abx116248 | Abbexa |
|
|
![]() Transmembrane Protein 70 (THEM70) Antibody |
|||
20-abx116249 | Abbexa |
|
|
![]() Transmembrane Protein 38B (TMEM38B) Antibody |
|||
abx122540-100ug | Abbexa | 100 ug | EUR 391 |
![]() Transmembrane protein 14B Antibody (HRP) |
|||
20-abx108836 | Abbexa |
|
|
![]() Transmembrane Protein 14B (TMEM14B) Antibody |
|||
20-abx110290 | Abbexa |
|
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![]() Transmembrane Protein 70 (TMEM70) Antibody |
|||
20-abx124676 | Abbexa |
|
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![]() Transmembrane Protein 43 (TMEM43) Antibody |
|||
20-abx318165 | Abbexa |
|
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![]() Transmembrane Protein 186 (TMEM186) Antibody |
|||
20-abx318180 | Abbexa |
|
|
![]() Transmembrane Protein 174 (TMEM174) Antibody |
|||
20-abx318456 | Abbexa |
|
|
![]() Transmembrane Protein 19 (TMEM19) Antibody |
|||
20-abx318457 | Abbexa |
|
|
![]() Transmembrane Protein 41A (TMEM41A) Antibody |
|||
20-abx318458 | Abbexa |
|
|
- HIgR/PRR1, able to inhibiting an infection, was mapped to the V area. Second, a soluble type of HIgR/PRR1 consisting of the only V area competed with cell-bound full-length receptor and blocked virion infectivity.
Recombinant human Ankyrin repeat domain-containing protein 30A
- Third, the V area was enough to mediate HSV entry, as an engineered type of PRR1 through which the 2 C2 domains had been deleted and the V area was retained and fused to its ransmembrane and cytoplasmic areas was nonetheless capable of confer susceptibility, though at decreased effectivity relative to full-length receptor. Constantly, switch of the V area of HIgR/PRR1 to a functionally inactive structural homologue generated a chimeric receptor with virus-entry exercise.
- Lastly, the only V area was enough for in vitro bodily interplay with gD. The in vitro binding was particular because it was competed each by <em>antibodies</em> to the receptor and by a mAb to gD with potent neutralizing exercise for HSV-1 infectivity.